Expert Answer:formal lab report: HPL analysis of Caffeine in mul

Answer & Explanation:Write formal lab report for( HPLC analysis of Caffeine in multiple products) expmake sure to write good paper




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For an AWESOME WONDERFUL lab report!
Grammar/Format: Double space, 12 ptfont, Times New Roman. Please re-read you
lab and make sure that your sentences make sense. Make sure that your format is logical and
follows a traditional paper: (Abstract)Intro, Experimental, Results/discussion,
conclusionetc. Your figures/graphs/tables should all be imbedded into the paper not
attached as “extra information”. Don’t forget to superscript and subscript and use units and
symbols! 10-3 M LaCb or 1.45 ± 0.03 M H2S04 (hint: word has shortcuts for all these things,
learn how to use them)
Intro: Explain the instrumentation you are using and give a little background on the lab.
What does the instrument read? What info are you receiving from the instrument? What are
you doing in the lab? Why is it important or fun to do this? Think of me as a
sophomore with basic chemistry background, but no instrumentation background.
Experimental: Explain what youdid and how you did it. Ifyou preformed calculations,
what general equations did you use? Yes you are following an established protocol and you
should cite it, but remember you probably made changes to the protocol and those should be
described here.
Results/Discussion: Your data should go in this section and you should be talking about it.
Remember the rules for figures ofmerit-label everything so that I know what I am looking
at. What should it tell me? Please don’t use connecting lines on scatter plots, instead add a
linear trend line with the equation and R2 value displayed. Don’t forget your sig fig rules:
1.4567 ± 0.025436 M becomes 1.45 ± 0.03 M. Ifyour data is not in a format for you to
imbed in your report (i.e. papers printed directly from the instrument) label them in an
appendix and reference that inyourlabreport (see appendix table 1for…). ·
Conclusions: Each lab has a series of questions throughout the lab and those questions should
be answered in this section in paragraph format in a logical order. This shouldn’t look like a
general chemistry lab report with all the questions laid out. Explain what you did
and answer the question that is associated with it.Finally have a paragraphat the end
with a summaryofwhat you did in the lab from beginning to end and what results you got
from it.
Reminders: if you look something up add a bibliography and cite your sources. If you didn’t
finish a portion ofthe lab mention it and talk about why.
Your lab is supposed to tell a story. Pretend I wasn’t sitting in lab -what did you d6? I
should be able to make a reasonable attempt to repeat the lab from what you wrote and get
similar results.
If you have questions email me: or make arrangements with me for office hours.
General Rubric for All Lab reports: (This will be modified to meet specific
experiment circumstances)
1) Grammar, proper references, etc
2) Intro – Explain instrumentation
Points Earned
3) Experimental section
Results/Discussion/Data – Print graphs etc
including Figures of Merit from syllabus
4) Questions answered from lab/Conclusions
50 pts
Additional Rubric for Formal Reports
1) Grammar revision, proper tense, etc
2) Proper Abstract
3) Figures in proper format for journal
4) Proper section heading
5) References in proper format
25 points
Point Earned
HPLC analysis of Caffeine in multiple products
To accurately determine the exact concentration of caffeine in three different
items, Excedrin, Tea, and Coffee using standard addition to quantify; the main
purpose of this lab is to learn the workings of the HPLC machine.
As you are running through the experiment below make sure you are collecting all
the data you need to provide these items in your lab report. This may necessitate
some additional data collection
Figures of Merit Checklist:
1. Standard addition data
2. statistical data for standard addition analysis
3. Table of true concentrations (standards and pseudo-unknown), estimated
concentrations with standard deviations (from equations not repeat trials),
and RSD’s.
4. Sensitivity
5. Detection limit
6. Chromatogram, printed from Excel and in proper format for publication in
the ACS Journal Analytical Chemistry (Consult the Author Guidelines under
Submission & Review ( ))
Caffeine Standard (1 mg/mL in mobile phase, you make)
Instant Coffee
Lipton Tea Bag
HPLC System.
Make sure the XDB-CB 4.0×150 mm column is attached
Mobile phase: 55 mL acetonitrile, 2 mL triethyl amine, 2 mL glacial acetic acid
diluted to 1 L in a volumetric flask. Be sure to filter mobile phase through 0.45
micron filters and degas before running.
Set UV detector to 254 nm
Mobile phase flow rate: 1.50 mL/min
Caffeine Standard.
Accurately weigh out 100 mg of pure caffeine and dissolve it into 100
ml solvent. (0.102 g weighed out). You will add varying aliquots of this to
your unknowns to perform the standard addition.
A sample of Excedrin was prepared by dissolving 10 mg in 100 mL of
mobile phase solvent which made a 100 ppm solution. Filter 1mL through a
0.45 micron syringe filter into each of 4 sample vials. Then add an
appropriate aliquot of caffeine standard to each vial to add 0, 5, 10 and 50
ppm. Make not of the final volume in each vial so you can calculate the added
caffeine concentration for the standard addition curve and account for
dilution. This solution was then injected onto the column via 10 μL injections
for a total of four injections.
Lipton Tea.
A Lipton tea bag was brewed in 100 mL of boiling water for five
minutes with frequent stirring. After five minutes the tea bag was removed
and the tea was allowed to continually cool to room temperature. Five mL of
the tea was then transferred into each of 5 50 mL volumetric flasks. Then
add to the flasks enough caffeine standard to successively give 0, 5, 10, 50
and 100 ppm added caffeine and dilute all 5 flasks to the mark with mobile
phase solvent. Filter 1mL of each flask through a 0.45 micron syringe filter
into sample vials.
Instant Coffee.
12 g of instant coffee mix was dissolved into 300 mL boiling distilled
water. The solution was then allowed to cool to room temp before 5 mL was
transferred to a 100 mL volumetric flask and diluted to the mark. Set up
samples for standard addition just like you did for the tea (only at 100 mL
this time). Filter 1mL through a 0.45 micron syringe filter into sample vials.
Data collection
Each solution should be run through the HPLC machine with 10 μL injections.
Find the peak area for each addition.
Additional questions to answer in your report:
1) Comment (compare etc.,) on the different ways of making your solutions for the
Excedrin compared to the coffee and tea. Was one more work than the other? Which
data analysis was more cumbersome?

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